The AMR Multi-Partner Trust Fund (MPTF) was established in 2019 to combat the threat of AMR through strategic collaboration in the fields of human, animal, plant and environmental health. The MPTF has been supporting countries and global components promoting a One Health approach for AMR response and carrying out National Action Plans (NAPs) on AMR. In 2024, the MPTF supported activities in ten countries and two components of the Global Programme. This annual report compiles and presents a summary of activities and outputs of both MPTF-supported country projects and Global Programme components in 2024 by MPTF Results Matrix Output. This annual report also highlights the progress made by the MPTF in 2024.